Stamping

From FreeBio

1. Backdilute cell cultures to OD600 = 0.1 - 0.3 (0.2 seems about ideal)

2. Concentrate sender culture 100X (1 ml --> 10 ul), concentrate receiver culture 10x (1 mL --> 100 ul)

3. Make M9 Agar slides by pipetting 300 ul M9 Agarose onto a slide.

4. Working in the tissue culture hood, ink the stamps by pipetting culture onto the posts. For the 1 mm stamps, I like to put 1 ul onto the square posts, and 5 ul onto the line. Let stamps dry for a few minutes.

5. Lift coverslip off of slide with razor blade. Stamp slides by gently dropping stamps onto the agar; wait approximately 10 seconds and lift stamp off.

6. Let slides dry for a few minutes.

7. Replace coverslip.