Sender-Receiver

From FreeBio

SENDER-RECEIVER EXPERIMENT

1. Grow up overnight 5 mL of I13272[Reciever] and PL - F1610[Sender]
2. Back dilute a stock of sender and receiver to OD 0.1 and grow up for 2 hours.
3. Make two negative control stocks of 1 mL sender, 1mL receiver, a positive control of receiver with 500nM AHL, and experiment tubes.
4. For the experiment tube, pellet 8.5 mL of sender, and resuspend in 1 mL receiver stock.
5. Grow together for 2 hours.
6. Watch.