SBC 2004
From FreeBio
In the summer of 2004, students at Boston University, Caltech, MIT, Princeton University, and The University of Texas at Austin worked to design and build genetically encoded finite state machines using standard, interchangeable biological parts. The students and instructors attended a Jamboree in November to share results and celebrate their work. Various articles have appeared about the summer competition and some of the projects explored during the competition have become publishable results.
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General
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Boston University
A team of students from Boston University and Harvard are continuing to build a chemical stimulus counter. The Coli Counter attempts to store the stimulus count in the DNA of E. Coli.
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Caltech
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MIT
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Princeton University
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A synthetic multicellular system for programmed pattern formation
- Subhayu Basu, Yoram Gerchman, Cynthia H. Collins, Frances H. Arnold and Ron Weiss
- Nature 434, 1130-1134 (28 April 2005)
- Pattern formation is a hallmark of coordinated cell behaviour in both single and multicellular organisms1, 2, 3. It typically involves cellcell communication and intracellular signal processing. Here we show a synthetic multicellular system in which genetically engineered 'receiver' cells are programmed to form ring-like patterns of differentiation based on chemical gradients of an acyl-homoserine lactone (AHL) signal that is synthesized by 'sender' cells. In receiver cells, 'band-detect' gene networks respond to user-defined ranges of AHL concentrations. By fusing different fluorescent proteins as outputs of network variants, an initially undifferentiated 'lawn' of receivers is engineered to form a bullseye pattern around a sender colony. Other patterns, such as ellipses and clovers, are achieved by placing senders in different configurations. Experimental and theoretical analyses reveal which kinetic parameters most significantly affect ring development over time. Construction and study of such synthetic multicellular systems can improve our quantitative understanding of naturally occurring developmental processes and may foster applications in tissue engineering, biomaterial fabrication and biosensing.
Also noted by Neil Halelamien on Slashdot
- Dynamic Control in a Coordinated Multi-Cellular Maze Solving System [paperplaza.net]
- Hsu, Allen (Princeton Univ.), Vijayan, Vikram (Princeton Univ.), Fomundam, Lawrence (Univ. of Maryland, Baltimore County), Gerchman, Yoram (Princeton Univ.), Basu, Subhayu (Princeton Univ.), Karig, David (Princeton Univ.), Hooshangi, Sara (Princeton Univ.), Weiss, Ron (Princeton Univ.)
- 2005 American Control Conference
- Control system theory provides convenient tools and concepts for describing and analyzing complex cell functions. In this paper we demonstrate the use of control theory to forward-engineer a complex synthetic gene network constructed from several modular components. Specifically, we present the design and simulation of a synthetic multi-cellular maze-solving system. Here, bacterial cells are programmed to use artificial cell-to-cell communication and regulatory feedback in order to illuminate the correct path in a user-defined maze of cells arranged on a surface. Simulations were used to analyze the system's spatiotemporal dynamics and sensitivity to various kinetic parameters. Experiments with Escherichia coli were carried out to characterize the diffusion properties of artificial cell-to-cell communication based on bacterial quorum sensing systems. The rational design process and simulation tools employed in this study provide an example for future engineering of complex synthetic gene networks comprising multiple control system motifs.
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