Building protein crystals

From FreeBio

Building Protein Crystals by Engineering Methods

ALife Boston talk by Julie Norville

It is difficult to build crystals for many proteins. I have a plan that might improve the process. The idea is to use a two-dimensional lattice of an easily crystallized protein as a substrate to which a protein of interest can be attached at identical lattice positions. The two-dimensional template will provide the lattice spacing and determine standardized dimensions for the unit cell of the crystal. The process can also be extended to create membrane protein crystals, for either a solubilized protein of interest or one that is embedded in a lipid layer.

References

  1. S.A. Darst, E.W. Kubalek, and R.D. Kornberg, 3-dimensional structure of Escherichia-coli RNA-polymerase holoenzyme determined by electron crystallography. Nature, 340(6236):730-732, 1989
  2. C.B. Mao, D.J. Solis, B.D. Reiss, S.T. Kottmann, R.Y. Sweeney, A. Hayhurst, G. Georgiou, B. Iverson, and A.M. Belcher. Virus-based toolkit for the directed synthesis of magnetic and semiconducting nanowires. Science, 303(5655):213-217, 2004
  3. K. Murate, K. Mitsuoka, T. Hirai, T. Walz, P. Agre, J.B. Heymann, A. Engel, and Y. Fujiyoshi. Structural determinants of water permeation through aquaporin-1. Nature, 407(6804):599-605, 2000
  4. U.B. Sleytr, B. Schuster, and D. Pum. Nanotechnology and biomimetics with 2-d protein crystals. IEEE Engineering in Medicine and Biology Magazine, 22(3):140-150, 2003
  5. H. R. Saibil. Macromolecular structure determination by cryo-electron microscopy. Acta Cryst., D56: 1215-1222, 2000.


Addendum: 3-4 Angstrom (high resolution) structures are possible (see Reference 5.)