BioSketch References
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TS Repressors
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LacI(ts)
- Andrews et al. 1996. A tightly regulated high level expression vector that utilizes a thermosensitive lac repressor: production of the human T cell receptor Vβ5.3 in Escherichia coli. Gene 182: 101-109. pdf
- Basu et al. 2005. A synthetic multicellular system for programmed pattern formation. Nature 434: 1130-1134. p {Designs several concentration band filters.}
- Bukrinsky et al. 1988. Multcopy expression vector based on temperature-regulated lac repressor: expression of human immunodeficiency virus env gene in Eschericha coli. Gene 70: 415-417. pdf {The ts lacI in this paper is cited by Hasan & Szybalski, 1995 and Andrews et al., 1996, but the identity of the mutation is not disclosed. It would be good if someone would find out.}
- Chao et al. 2002. Construction and characterization of thermo-inducible vectors derived from heat-sensitive lacI genes in combination with the T7 A1 promoter. Biotechnol Bioeng 79: 1-8 link
- Elowitz & Leibler. 2000. A synthetic oscillatory network of transcriptional regulators. Nature 403: 335-338. link {Uses tightly repressible promoters of cI and lacI.}
- Hasan & Szybalski. 1995. Construction of lacIts and lacIqts expression plasmids and evaluation of the thermosensitive lac repressor. Gene 163: 35-40. link {Documents temperature sensitive lac repressors.}
- Wang et al. 2004. Improvement of the thermoregulated T7 expression system by using the heat-sensitive lacI. Biotechnol Prog 20: 1350-1358. link
- Yabuta et al. 1995. Thermo-inducible expression of a recombinent fusion protein by Escherichia coli lac repressor mutants. J Biotechnol 39: 67-73. link
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CI857
- Gardner et al. 2000. Construction of a genetic toggle switch in Escherichia coli. Nature 403: 339-342. link {Controls CI857 degradation by raising temperature.}
- Slavcev et al. 1998. Acquired mutations in phage lambda genes..., Gene, 223, 115-118. [1]Jenny 20:19, 12 Jun 2005 (EDT)
- Lubitz et al. 1999. Altered Temperature Induction Sensitivity..., FEMS MICROBIOL LETTERS, 173, 347-352. [2]Jenny 20:19, 12 Jun 2005 (EDT)
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Others
- Irr & Englesberg. 1971. Control of expression of the L-arabinose operon in temperature-sensitive mutants of gene araC in Escherichia coli B/r. J Bacteriol 105: 136-141. link
- Vogel et al. 1991. Temperature-sensitive mutations in the bacteriophage Mu c repressor locate a 63-amino-acid DNA-binding domain. J Bacteriol. link {Gives induction times.}
- Wissmann et al. 1991. Selection for Tn10 Tet repressor binding to tet operator in Escherichia coli: isolation of temperature-sensitive mutants and combinatorial mutagenesis in the DNA binding motif. Genetics 128: 225-232. pdf
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SOS Response
- Higashitani et al. 1995. SOS induction in Escherichi coli by single-stranded DNA of mutant filamentous phage: monitoring by cleavage of LexA repressor. J Bacteriol 177: 3610-3612. pdf
- Yasuda et al. 1998. Inhibition of Escherichia coli recA coprotease activities by DinI. EMBO J 17: 3207-3216. link
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CI Repressor
- Kobayashi et al. 2004. Programmable cells: interfacing natural and engineered gene networks. PNAS 101: 8414-8419. link {Uses the SOS, UV-response pathways as well as the AHL-mediated quorum sensing pathways.}
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434 Repressor
- Pawlowski & Koudelka. 2004. The preferred substrate for RecA-mediated cleavage of bacteriophage 434 repressor is the DNA-bound dimer. J Bacteriol 186: 1-7. link
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Toggle Switch
- Gardner et al. 2000. Construction of a genetic toggle switch in Escherichia coli. Nature 403: 339-342. link {Implements the basic toggle switch that is at the heart of the BactoSketch.}
- Guet et al. 2002. Combinatorial synthesis of genetic networks. Science 296: 1466-1470. link {Uses cI and lacI repressors.}
- Wolf & Arkin. 2003. Motifs, modules and games in bacteria. Curr Opin Micobiol 6: 125-134. link
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Methods
- Cotransformation of E. coli: link
