BioSketch BioBricks

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These are the parts that we wanted from the Registry. Note: we didn't get all of them

• Lambda cI
  • BBa_C0051: cI(LVA) [750bp] in pSB1A2 [2079bp], DH10B cell
  • BBa_C0081: cI-857(LVA) [750bp]; building
  • BBa_R0051: P(lambda-cI) [49bp] in pSB1A2 [2079bp] DH10b cell

• 434 Repressor
  • BBa_C0052: 434(LVA) [669bp] in pSB1A2 [2079bp], DH10B cell
  • BBa_R0052: P(434) [46bp] in pSB2K3 [4425bp], DH5a cell

• LacI
  • BBa_C0012: LacI(LVA) [1128bp] in pSB1A2 [2079bp], DH10B cell
  • BBa_R0010: P(lacI_can) [200bp] in pSB1A2 [2079bp], DH10B cell
  • BBa_R0011: P(lacI_hyb) [55bp] in pSB1A2 [2079bp], DH10B

• Reporters
  • BBa_E2060: mCherry(yeast) [744bp] in N/A
  • BBa_E2030: venusYFP(yeast) [753bp] in N/A
  • BBa_E0040: GFPmut3b [720bp] in N/A

• Assembled Reporters (includes RBS BBa_B0034k, reporter, and T BBa_B0015)
  • BBa_I13504: GFP (mut3b) [857bp] in N/A

• Cells
  • BBa_V1002: MC4100(LacI-)

• RBSs
  • BBa_B0030: RBS(strong) [15bp] in pSB1A2 [2079bp], DH10B cell
  • BBa_B0034: RBS(extraStrong) [12bp] in pSB1A2 [2079bp], DH10B cell

• Terminators
  • BBa_B0015: T(double): forward efficiency of 0.984 [129bp] in pSB1AK3 [4425bp], DH5a cell

• Inverters (includes RBS BBa_B0034, gene, T BBa_B0015, and promoter)
  • BBa_Q04121: LacI using LacI pL [1370bp] in pSB2K3 [4425bp], D1210 cell
  • BBa_Q04122: LacI [1516bp] PLANNING
  • BBa_Q04510: Lambda cI [987bp] in pSB2K3 [4425bp], D1210 cell
  • BBa_Q04520: 434 [903bp] in pSB2K3 [4425bp], D1210 cell
  • BBa_Q04811: 857 [962bp] PLANNING

• Primers (forward/reverse)
  • BBa_G00120/BBa_G00121: for BBa_C0012
  • BBa_G00510/BBa_G00511: for BBa_C0051
  • BBa_G00520/BBa_G00521: for BBa_C0052

• Notes on vectors & strains
  • pSB1A2: high-copy (100-300/cell) plasmid w/ AmpR
  • pSB1AK3: high-copy (100-300/cell) plasmid w/ AmpR and KanaR
  • DH5a aka BBa_V1001
  • DH10B aka V1004