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|My perl script for characterization of the human chromosome 11 is [http://karma.med.harvard.edu/wiki/Perlscr#DNA_characterization_version_1.1 here].||My perl script for characterization of the human chromosome 11 is [http://karma.med.harvard.edu/wiki/Perlscr#DNA_characterization_version_1.1 here].|
|+||==What if we had a 1000 genomes==|
|+||If we had a 1000 diploid human genome sequences, what would we do?|
|+||# Establish the normal genome sequence - that is which nucleotides are most common at each position. How does this compare with the present Human Genome draft.|
|+||# Conservation of each individual nucleotide - which are most variable.|
|+||# How does well characterized categories like: extrons, introns, known transcription factor binding sites, siRNA, and what is still called 'junk' DNA etc. map onto the variability map. For instance are extrons primarily in regions of low variability whereas introns are in regions of high variability?|
|+||# For all protein coding DNA frames how are amino acid sequences conserved.|
|+||==Interesting organisms for biodiesel metabolic engineering==|
|In the search for some enzymes that metabolize octane I have found the following organisms, that are worth looking further into:||In the search for some enzymes that metabolize octane I have found the following organisms, that are worth looking further into:|
|*Gloeobacter violaceus||*Gloeobacter violaceus|
Revision as of 17:46, 8 November 2005
Morten Sommer wiki page
101 Week 8
My perl script for characterization of the human chromosome 11 is here.
What if we had a 1000 genomes
If we had a 1000 diploid human genome sequences, what would we do?
- Establish the normal genome sequence - that is which nucleotides are most common at each position. How does this compare with the present Human Genome draft.
- Conservation of each individual nucleotide - which are most variable.
- How does well characterized categories like: extrons, introns, known transcription factor binding sites, siRNA, and what is still called 'junk' DNA etc. map onto the variability map. For instance are extrons primarily in regions of low variability whereas introns are in regions of high variability?
- For all protein coding DNA frames how are amino acid sequences conserved.
Interesting organisms for biodiesel metabolic engineering
In the search for some enzymes that metabolize octane I have found the following organisms, that are worth looking further into:
- Gloeobacter violaceus
- Nocardia farcinica
- Mycobacterium bovis
- Mycobacterium tuberculosis CDC1551
- Mycobacterium tuberculosis H37Rv
- Burkholderia sp. 383
- Burkholderia pseudomallei 1710b
- Burkholderia mallei
101 Week 7
Here is my perl script :
New study about economic cost of global climate change from harvard 
Some interesting enzymes/organisms metabolizing octanes:
- Alkane hydroxylases 
- Octane 1,3 diol 
- Octane metabolizing organisms: Corynebacterium sp. Strain 7E1C*, Pseudomonas oleovorans (Alk operon has been characterized in mid 90'ies).
101 Week 6
Octane producing photosynthetic organism
It would be neat if we could engineer an organism that produces octane (gasoline) from sunlight to complement the biodiesel. The enzymatic reaction performed by alkane hydroxylase may be of interest .
In silico organisms for metabolic network analysis
- Another useful resource is the Kyoto Encyclopedia of Genes and Genomes KEGG which has listed metabolic pathways of many organisms including an extensive database of ligands, enzymes, reactions etc.
The rutine OptStrain is likely to prove highly useful for maximizing the biofuel production of a suitable organism.
The OptStrain rutine consist of the following 4 simple steps:
- Compile Universal Database of all comfirmed enzyme catalyzed reactions
- Calculate maximum theoretical yield of product given a particular substrate input using all reactions available in Universal Database
- Identify a pathway that maximizes yield of product while minimizing number of non native functionalities
- Optimize the resulting metabolic network by knocking out enzymes that direct flux away from the product pathway. (OptKnock) - See Jeremy description
101 Week 5
Metabolic Engineering and Optimization
I have been writing the initial draft outline for the biodiesel project and have been looking at Jeremy's metabolic engineering tutorial and the Pallson review paper PDF to familiarize myself with the field.
An interesting approach to metabolic engineering is bilevel optimization in which the bright researcher modifies the genome of the production organism in such a way that the organism in optimizing its biomass production will produce a particular compound as a biproduct/wasteproduct. This method has been applied by the [Maranas group] at Penn State to vaious processes including the production of Hydrogen, particular amino acids and vannilin.
The figure on the right illustrates the process of bilevel optimization for the case of hydrogen production. The organism will naturally seek to optimize its biomass production shown on the x axis given its particular genotype. When particular pathways are blocked or added the organism the organism may not grow as fast as the wildtype organism; however, a result of the modified pathways is the accumulation of a pÃ¥articular reaction product in this case hydrogen. The total hydrogen production that one wishes to maximize is thus given as the product of the biomass production rate times the fraction of the biomass that is hydrogen. From the example on the figure it is seen that Wildtype E.Coli does not produce significant hydrogen; anaerobic E. Coli produces 4.5 mmol/g DW wildtype biomass in an hour; the double knock out produces 16.25 mmol/g DW wildtype biomass; the triple knock out produces 4.8 mmol/g DW wildtype biomass. Thus, significant increases in hydrogen production can be acheived by making a few deletions in the genome of E. Coli.
Bilevel optimization references
- Pharkya et.al. Genome Research (2004) 14:2367 []
- Burgard et.at. BIOTECHNOLOGY AND BIOENGINEERING (2003) 84:647 []
- Burgard et.al. Genome Research (2004) 14:301 []
101 Week 4
Metrics for biofuels
In order to quantitatively evaluate different options of fuels comparable metrics need to be established. I am trying to list a couple, but please fill in--Msommer 00:17, 12 October 2005 (EDT).
- Fossil fuel energy balance. The ratio of the energy output to the fossil energy input. further info
- Energy density. (J/L)
- Required infrastructure investment. ($) The investment required to set up infrastructure to distribute the fuel and develop engines/applicances that utilize the fuel.
- Net CO2 emmission pr energy unit. (g/J) The net amount of carbon dioxide emitted to the atmosphere from the active production and combustion of x energy units of fuel. active production refering to fuels that are actively produced on a reasonable timescale like alchol from corn or biodiesel from algae.
- Emmission pr energy unit of other substances. Such as: CO,SO2,NO2page 1773, section 1.2 and 1.3
- Cost of production/preparation per energy unit. ($/J) The cost of production of an energy unit of the final fuel.
- The first derivative w/r/t time of the above ($/Jmonth). (this to "Ratio..." --Jleith 08:45, 13 October 2005 (EDT))
- The estimated cost of production/preparation per energy unit averaged over the next ten years, or thirty years, etc.
- Proportion of money paid for a fuel that ends up in the hands of regimes or organizations unfriendly or hostile to the U.S. (or to the West, etc.).
- Ratio of humans' ability to produce this fuel to the worldwide demand for the fuel.
Fuel metric table
Please fill in if you find appropriate information including a link to the source
--Msommer 23:22, 12 October 2005 (EDT)
|Fosil fuel energy balance||0.805 1||0.843 1||1.34 1||3.20 1|
|Energy density. (J/L)||34280000 2||39296000 2||?||35395000 2|
|Required infrastructure investment ($)||0||0||?||?|
|Net CO2 emmission pr energy unit (g/J)||?||?||?||?|
|Cost of production/preparation pr energy unit US prices ($/GJ)||21.9 Oct 10 2005||21.2 Oct 10 2005||?||5.2 do it yourself - veg. oil - 14.0 2002 commercial prices|
|Cost of production/preparation pr energy unit Danish prices ($/GJ)||44.7 Oct 12 2005||42.2 Oct 12 2005||?||?|
|Sustainable fuel production capacity / Global fuel need||?||?||?||?|
Links regarding (renewable) energy
- National Renewable Energy Laboratory webpage contains good links to various reports on renevable energy sources.
- Monthly Energy Review is a good journal for various stats on energy consumption, pricing etc.
- This study states that biodiesel emmission of CO is not significantly different from ordinary diesel.
- Biodiesel Community is a webpage tutorial on how to make biodiesel from vegitable waste oil
- wikipedia entry references several good studies
- The University of Idaho Biodiesel Group has performed several studies of biodiesel emmission and commercial viability.
- The biodiesel warehouse is one among many companies that offer equipment for people to produce their own biodiesel from waste vegetable oils.
- Energy Information Administration on biodiesel - critical paper on concerned with the commercial viability of large scale biodiesel from soybean oil.
101 Week 3
Economics of a Sustainable Environment
Planet Earth is a constrained environment with fixed amounts of natural resources. Making sure that natural resources (clean water, minerals etc.) and services (such as oxygen production etc.) will be available to future generations of humans require moving towards an economy that appreciates the real value of these resources. Real value meaning the cost of renewal NOT the cost of extraction. An economy of this kind has been termed 'Natural Capitalism' by Paul Hawken, Amory Lowins and L Hunter Lovins in their book 'Natural Capitalism'.
The central paradigm of Natural Capitalism is that:
- Planet Earth should not be depleted for resources at a rate higher than the rate at which resources are generated and waste should not be generated at rates higher than it can be reabsorbed by the ecosystem
- Limit use of all resources to rates that ultimately result in levels of waste that can be absorbed by the ecosystem
- Exploit renewable resources at rates that do not exceed the ability of the ecosystem to regenerate the resources
- Deplete nonrenewable resources at rates that, as far as possible, do not exceed the rate of development of renewable alternatives
Most researchers agree that the state of the global environment is critical; however, for a different view check out BjÃ¸rn Lomborg and his book 'The Skeptical Environmentalist'. In this book he argues that the state environment of the environment is not too bad after all. This book resulted in immense criticism from various experts and acqusations of use of non scientific methods; however, it also had support from various sources.
I beleive that it is important that we take into account these issues in our projects. With respect to the Biodiesel project this is likely to be fulfilled, since biological systems are usually extremely good at cycling resources through the ecosystem without buildup of waste deposits. However, we must fully investigate and understand the pertubations made to the ecosystem by introducing billions of billions of algea into particular locations on the earth as part of the project. A powerful demonstration of the usefulness of genetic engineering would be to engineer the biodiesel producing algea in such a way that a full energy production/consumption cycle did not perturb the ecosystem. --Msommer 12:38, 2 Oct 2005 (EDT)
Copenhagen Consensus - Set priorities for planet Earth
Copenhagen Consensus is an experiment performed by the economist BjÃ¸rn Lomborg in May 2004. A panel of eight expert economist (including three nobel laureates) were suposed to make a cost/benefit analysis of how to prioritize funding for 10 major global challenges. The global challenges were: Communicable diseases, Malnutrition and hunger, Subsidies and trade barriers, Climate change, Conflicts, Education, Financial instability, Government and corruption,Population: migration, Sanitation and water. For each of the challenges a set of experts had compiled a report that included a analysis of the challenge, suggested actions (and their cost and benefit). Based on these reports the eight experts had 4 days of round table discussion leading to a prioritization of the challenges.
The Copenhagen Consensus was an exercise in global prioritization and is in my opinion highly relevant for 101. More info can be found at The copenhagen consensus homepage. Furthermore a book has been written about the project including summaries of the reports decribing the challenges. The book is 'Global Crises, Global Solutions.
In my oppinion the outcome of the project seems to neglect the environmental challenges faced by our generation - However, the idea behind the project and the methods are highly relevant and recommendable.--Msommer 12:38, 2 Oct 2005 (EDT)
101 week 2
To be considered in the project description
Irespectively of what system we choose to model such as the biodiesel idea I think that it will be important to consider the economical flux of capital (money, bio capital, labor and profit) from a global perspective. To take the example of biodiesel production: How will the source of funding (both in terms of nations and within nations: public versus private) affect the econmical outcome of such a project globally. That is if a project is initially funded by the US government followed by a commercialization through private US companies, how will it differ from a internationally funded public coorporation? (If such a scenario is possible!) How should we make quantitative graphical representations for this? Here is a suggestion for what that may look like. --Msommer 20:31, 28 Sep 2005 (EDT)
Complexity & Randomness
Trying to define what is random: a collection of elements is random when the correlation of the elements behavior/value is unpredictable - however, the properties of the total system may be straight forward to describe. Ex.: A random string of numbers between 1 and 9. There should be no correlation between the number at position a and a+k, but the average value of all the numbers will be appr. 5.
Trying to define a complex system: a collection of elements is complex if the correlation of the elements behavior/value is non trivial, but predictable. As complexity increases determining the underlying correlations become more difficult. The total complex system may exhibit non trivial behavior. Ex. Weather. Or simple behavior, when E.Coli responds to lactose by switching on the lac genes.
My understanding of 101
As I understand the objective of this class, we should try and develop quantitative models that can be used to make qualified policy decisions that impact our life and our world - focus being on technology and research. These models should integrate knowledge from all levels - in a sense showing the relevance of a particular reaction or technological advance to society. This seems in some aspects as a tremendous task - and I think that it is important to establish which 'societal' parameters that we wish to link to the lower level processes. Is it: bio capital, average lifetime, public health and GDP. Furthermore, I think that it will be important to take into consideration that most decisions are not based solely on rationality, but also moral and self interest. So if one wishes to develop tools for decision makers - in order for them to make rational decisions - a framework must be put together minimizing effects of non rational considerations (or at least disclosing them). --184.108.40.206 18:38, 21 Sep 2005 (EDT)
I am a Biophysics 1 year PhD student. I was born in Denmark and have lived there most my life except for some periods as an exchange student in Pasadena, California. I finished my M.Sc. in Physics and Biophysics Fall 2004 and have been working on microfluidic technology development for protein crystallography before I started at Harvard - I also cofounded a company doing rational approaches to protein crystallization called Formbion. I have been very interested in issues regarding society, since I was young, but during my university studies I have not been spending all the time I would have liked to thinking about these issues. Hence, I welcome this course as an opportunity for spending some more time on these subjects.