BioWire BioBricks

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Contents


Part Description Length Order Date Arrival Date Plasmid ID Miniprep status
F1611 Sender construct, ordered before, not received. Tuesday, June 21st
C0161 needed for building F1611 Tuesday, June 21st
E0434 eYFP with AAV degradation tag instead of LVA degradation tag (slower half-life?) Tuesday, June 21st
P0479 twin of I0464, might not be in the box that has gone missing? Tuesday, June 21st
C0060 aiiA, LVA Tuesday, June 21st
C0061 luxI, LVA Tuesday, June 21st
C0160 aiiA, LVA- Tuesday, June 21st
C0161 luxI, LVA- Tuesday, June 21st
I0460 aiiA with Elowitz RBS and terminator, LVA . Tuesday, June 21st
P34160 aiiA with Elowitz RBS and terminator, LVA-. Tuesday, June 21st
B0035 RBS (non-standard Shine Dalgarno sequence) 8bp Monday, June 13th
F1611 Missing, but we have subparts (B0034, B0015) and ordered (C0161) Monday, June 13th
B0034 subpart of F1611; RBS (Elowitz 1999) 12bp Monday, June 13th Friday, June 17th pSB1A2 OK
C0161 Need to order this one
B0015 subpart of F1611; terminator Monday, June 13th Friday, June 17th pSB1AK3 OK
I13272 Missing, we have subparts for subpart F2621 and might replace E0434 with E0432 Monday, June 13th
F2621 Missing, but we have subparts (R0062, R0063, I0462) Monday, June 13th
subpart R0062 subpart of F2621 Monday, June 13th Friday, June 17th pSB1A2 OK
subpart R0063 subpart of F2621 Monday, June 13th Friday, June 17th pSB1A2 OK
subpart I0462 subpart of F2621 Monday, June 13th Friday, June 17th pSB1A2 OK
E0434 replacing with E0432 in test construct, will order soon Monday, June 13th
I13602 Missing, but we have subparts (R0040, E0422) Monday, June 13th
R0040 subpart of I13602 Monday, June 13th Friday, June 17th pSB1A2 OK
E0422 PREVIOUS ORDER (subpart of I13602) 917bp Monday, June 13th Tuesday, June 14th pSB1A2 OK
B0030 Monday, June 13th Friday, June 17th pSB1A2 OK
B0031 RBS.2 (weak) 14bp Monday, June 13th Friday, June 17th pSB1A2 OK
B0032 RBS.3 (medium) 13bp Monday, June 13th Friday, June 17th pSB1A2 OK
B0033 RBS.4 (weaker) 11bp Monday, June 13th Friday, June 17th pSB1A2 OK
C0051 Monday, June 13th Friday, June 17th pSB1A2 OK
C0070 Enzyme, rhlI (LVA ) Autoinducer synthesis protein that produces N-butyryl-HSL which binds to RhlR, obtained from Pseudomonas aeruginosa. Monday, June 13th Friday, June 17th pSB2K3 OK
C0071 Repressor/Activator, rhlR (LVA ) Transcriptional regulator, in complex with N-butyryl-HSL, RhlR binds to the Rhl promoter Arrived Monday, June 13th Friday, June 17th pSB2K3 OK
C0078 coding region for lasI protein, which produces the chemical signal AI-1 Arrived pSB2K3 Monday, June 13th Friday, June 17th pSB2K3 failed
C0079 coding region for lasR protein, which accepts chemical signal AI-1 (made by lasI protein) Monday, June 13th Friday, June 17th pSB2K3 OK
E0032 Monday, June 13th Friday, June 17th pSB1A2 OK
R0065 Monday, June 13th Friday, June 17th pSB1A2 OK
R0079 LasR promoter Monday, June 13th Friday, June 17th pSB2K3 failed
I0464 Produces LasR protein which can sense AI-1 in the media and activate transcription from R0078, the right hand Lux promoter

Missing, but we have all the subparts (B0034, C0079, B0015)

Monday, June 13th
I14015 LasR and 3OC12HSL regulated promoter with a single Tet Operator (binding site for TetR repressor) Monday, June 13th Friday, June 17th pSB2K3 OK
I14016 LasR and 3OC12HSL regulated promoter with CI Operator 1 (binding site for lambda cI repressor) Monday, June 13th Friday, June 17th pSB2K3 OK
I14017 Rhl promoter Monday, June 13th Friday, June 17th pSB2K3 OK
I14018 Constitutive promoter? Monday, June 13th Friday, June 17th pSB2K3 failed
F1610 798bp Thursday, June 9th Tuesday, June 14th pSB1AK3 OK (failed 1st time)
E0040 720bp Thursday, June 9th Tuesday, June 14th pSB1A2 OK
E0420 878bp Thursday, June 9th Tuesday, June 14th pSB1A2 OK
E0422 917bp Thursday, June 9th Tuesday, June 14th pSB1A2 OK (failed 1st time)
E0430 878bp Thursday, June 9th Tuesday, June 14th pSB1A2 OK
E0432 917bp Thursday, June 9th Tuesday, June 14th pSB1A2 OK (failed 1st time)


Under Consideration

Part Thoughts
C0060 Lactonase? Talked about on Friday, June 17
BBa_I14023 Constitutive LasR (Planning)
BBa_I14024 Constitutive RhlR (planning)


Original list

No, I'm not sure why this is still here, except that I don't like deleting things.

Weiss Sender, parts 1-2 (of 3)

  • [ordered]BBa_F1610 -- 3OC6HSL Sender Device. This is the luxI, linked to a promoter, used to duplicate the Weiss circuit.
  • BBa_R0040 -- Promoter (tetR, negative). This is the PLtet0-1 promoter that gets hooked up to the HSL sender biobrick
  • BBa_I13017 -- TetR under Plac control. This makes the constitutive promoter of tetR.  ?We want to ask if this works?

Weiss Recevier, parts 1-2 (of 3)

  • [ordered]BBa_F2621 -- 3OC6HSL Receiver Device. We're not sure why the promoter needs to be regulated, instead of being constitutively present.
  • put an RBS in
  • BBa_C0051 -- Repressor, Lambda cI (RBS- LVA ). This is the CI(LVA)
  • BBa_B0015 -- Double terminator consisting of BBa_B0010 and BBa_B0012.

Weiss Receiver, part 3 (of 3)

  • R0065 BBa_R0065 -- Promoter (lambda cI and luxR regulated -- hybrid)
  • BBa_E0044 -- GFP-AAV.  ?Does anyone have better ideas for a reporter?

Ribosome Binding Sites

Also Ordered

  • [ordered]BBa_I13272 YFP Producer Controlled by 3OC6HSL Receiver Device
  • [ordered]BBa_I12224 LacI protein generator (LVA-)
  • [ordered]BBa_E0422 Reporter ECFP (RBS LVA TERM) (B0034.E0022.B0015)


Possible extra repressor

  • BBa_R0011 -- Promoter (lacI regulated, lambda pL hybrid) (actually, this is a backup repressor) Arrived pSB1A2